胶原蛋白杂交肽是3-Hisperson Inc.的产品。胶原蛋白杂交肽(CHP)是一种新型独特的肽,可在体外和体内特异性结合未折叠的胶原蛋白链[1 2 3]。通过共享天然胶原蛋白的Gly-X-Y重复序列,CHP具有通过以类似于退火至互补DNA链的DNA片段的方式重新形成三螺旋结构而与变性胶原蛋白链杂交的强能力。CHP非常具体:由于缺乏结合位点,它对完整的胶原蛋白分子具有可忽略的亲和力,并且由于其中性和亲水性,它对非特异性结合是惰性的。CHP是一种强大的组织病理学工具,能够直接检测由多种疾病引起的炎症和组织损伤以及发育和衰老期间的组织重塑[3]。CHP可在不使用合成荧光基质或遗传修饰细胞的情况下,在3D胶原蛋白培养物中通过癌细胞的蛋白水解迁移而稳健地可视化细胞周基质周转[4]。CHP可以在分子水平上测量和定位胶原组织的机械损伤[5]。它还能够评估脱细胞细胞外基质中的胶原蛋白变性[6]。此外,CHP可用于在SDS-PAGE凝胶中特异性地显示胶原蛋白条带,而不需要蛋白质印迹[7]。(目录号:BIO)用于抗生物素蛋白/链霉抗生物素蛋白介导的方法,包括HRP-酶法5-FAM(目录号:FLU),用于绿色荧光检测和Cy 3(Catalog #:RED)用于红色荧光检测。应用:免疫荧光免疫组化SDS-PAGE(凝胶内蛋白质印迹)运输条件室温下以粉末形式快速运输。储存-20 ° C作为粉末; 4 °C在水中重构后无需等分和冷冻。参考文献1)"Targeting and mimicking collagens via triple helical peptide hybridization“ Curr Opin Chem Biol 2013 17 968- 975. 2)”Targeting collagen strand by photo-triggered triple-helix hybridization“Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772.DOI:10.1073/pnas.1209721109.3)“用胶原杂交肽进行组织重塑的原位成像。ACS Nano 2017 11 9825。DOI:10.1021/acsnano.7b031504)“通过肽杂交可视化胶原蛋白水解:从3D细胞培养到体内成像。“Biomaterials 2018 DOI:10.1016/j.biomerials.2018.08.0395)“胶原杂交肽实现胶原中机械损伤的分子水平检测和定位。《国家通讯》 2017 8 14913 DOI:10.1038/ncomms14913.6)“使用胶原杂交肽对脱细胞组织中的胶原变性进行分子评估。生物材料学报 2017. 53 268-278 DOI:10.1016/j.actbio.2017.01.0797)“Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides” Bioconjugate Chemistry 2013 24 9-16. DOI:10.1021/bc3005842.8)“在肌腱中,不同的生理需求导致功能不同的纳米结构。“科学。2018年报告。DOI:DOI:10.1038/s41598-018-22741- 8蛋白酶-比酶谱法DQ胶原SHG和TEM更可靠、更方便-对胶原蛋白具有高亲和力和无与伦比的特异性,基本上没有非特异性结合-适用于所有物种的所有类型的胶原蛋白,依赖于胶原蛋白的二级结构,而不是任何定义的结合序列-一种非抗体方法,对任何共染色抗体没有物种限制-适用于冷冻和石蜡包埋切片,无需抗原检索-小尺寸(2%的IgG分子量),使组织渗透和整个标本染色变得容易,无需切片-在4 °C溶液中稳定,无需等分储存
产品描述
The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture without the use of synthetic fluorogenic matrices or genetically modified cells [4]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [5]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [6]. In addition CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [7].CHP come in three varieties: labeled with biotin (Catalog #: BIO) for avidin/streptavidin-mediated including HRP-enzyme methods 5-FAM (Catalog #: FLU) for green fluorescence detection and Cy3 (Catalog #: RED) for red fluorescence detection.Applications: immunofluorescence immunohistochemistry SDS-PAGE (in-gel Western blot)Shipping ConditionsExpress shipping as powder at ambient temperature. Store at -20 °C upon arrival until use.Storage-20 °C as powder; 4 °C after reconstitution in water no need to aliquot and freeze. Stable for at least 6 months in solution at 4 °C.References1) “Targeting and mimicking collagens via triple helical peptide assemblies†Curr Opin Chem Biol 2013 17 968-975.2) “Targeting collagen strand by photo-triggered triple-helix hybridization†Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772. DOI: 10.1073/pnas.1209721109.3) "In situ imaging of tissue remodeling with collagen hybridizing peptides." ACS Nano 2017 11 9825. DOI: 10.1021/acsnano.7b031504) "Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging." Biomaterials 2018 DOI:10.1016/j.biomaterials.2018.08.0395) "Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides." Nat. Commun. 2017 8 14913 DOI: 10.1038/ncomms14913.6) "Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide." Acta Biomater. 2017. 53 268-278 DOI: 10.1016/j.actbio.2017.01.0797) “Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides†Bioconjugate Chemistry 2013 24 9-16. DOI: 10.1021/bc3005842.8) "In tendons differing physiological requirements lead to functionally distinct nanostructures." Sci. Reports 2018. DOI: DOI:10.1038/s41598-018-22741-8Features-More informative reliable and convenient than zymography DQ collagen SHG and TEM-High affinity and unparalleled specificity to collagen with essentially no nonspecific binding-Applicable to all types of collagen from all species relying on collagen's secondary structure instead of any defined sequence for binding-A non-antibody approach with no species restrictions against any co-staining antibody-Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval-Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning-Stable in solution at 4 °C eliminating the need to aliquot for storage