This Control CHP is a scrambled version of the Target that does not bind to unfolded collagen.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo.[1 2 3] By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.The in vivo CHP incorporates our newest sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step. [4] Therefore the in vivo CHPs will always be in their active form and will not self-hybridize once injected. [4] The sCy7.5-CHP is a powerful in vivo tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging.[3] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5]The sCy7.5-CHPs are labeled with sulfonated-cyanine 7.5 dye for near-infrared fluorescence (NIRF) detection. This wavelength offers better tissue penetration while minimizing tissue autofluorescence.Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g. I II III IV etc).[3 6]Applications: in vivo imaging small animal imagingFeatures New sequence designed for in vivo use- does not need any pre-activation step unlike CHPs for histology Near-infrared imaging probe minimizes the chance of autofluorescence in the gut or certain tissues Can be used for systemic or in situ injection Signal lasts at target sites for days allowing for imaging at multiple time points with a single dose High affinity and unparalleled specificity to collagen turnover with essentially no nonspecific binding Applicable to all types of collagen from all species relying on collagen's secondary structure instead of any defined sequence for binding Stable in solution under 4 °C up to three months eliminating the need to aliquot for storageKey Publications1.Targeting and mimicking collagens via triple helical peptide assemblies. Curr. Opin. Chem. Biol. 2013. [link]2.Targeting collagen strands by photo-triggered triple-helix hybridization. Proc. Natl. Acad. Sci. U.S.A. 2012. [link]3.In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano 2017. [link]4.Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials 2018. [link]5.Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nat. Commun. 2017. [link]6.Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjug. Chem. 2013. [link]Additional Information sCy7.5-CHP dose can be adjusted based on animal model or application. Current dosing is based on a mouse model.
