9-叔丁基多西环素（9 TB）是一种泰特开/关系统激动剂，其能够激活四环素反式激活因子（tTA）和反向tTA（rtTA）以及tTA响应性启动子（Ptets）并充当其诱导物，在本文中称为泰特开关。该化合物能够以大约10倍的功效激活泰特开关，特别是在亲脂性环境如脑和肺以及其他类似的生物区室中。9 TB易溶于水，可以IV IP给药，也可以在实验动物的饮用水中给药，便于随时给药。9 TB还发现了使用四环素诱导（tet-on）系统研究缺氧调节的基因表达对细胞存活的作用，其中暴露于诱导配体多西环素（dox）抑制线粒体中的半胱天冬酶-3切割。2007; 2（6）：e533.2）Halterman M.W.“使用Tet-On系统研究凋亡相关基因的改进方法”J. Biomol.屏幕2011; 16（3）：332-3373）Jiang D.等人“Airway Epithelial NF-kB Activation Promotes Mycoplasma pneumoniae Clearance in Mice”PLoS ONE. 2012; 7（12）：e52969.4）Furutachi S.等人“Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells”Nature Medicine 2015; doi：10.1038/nn.3989.5）Furlan G. 等（2017）。“个体神经干细胞的终身神经原性活动和持续生长在硬骨鱼的软腭中建立了一种由外向内的结构。《当代生物学》27（21）：3288-3301。DOI：10.1016/j.cub.2017.09.0526）Alonso S. B。 等（2019）。“神经干细胞和嗅球成年神经发生的增加提高了对高度相似气味的辨别力。“The EMBO Journal 38：e98791. DOI：10.15252/embj.201798791
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9-tert-Butyl Doxycycline (9TB) is a Tet On/Off system agonist capable of activating and acting as an inducer for the tetracycline-transactivator (tTA) and reverse tTA (rtTA) and tTA-responsive promoters (Ptets) herein known as the Tet switch. The compound is able to activate the Tet switch with approximately 10-fold greater efficacy especially in lipophilic environments such as the brain and lung and other similar biocompartments. 9TB is freely soluble in water and can be administered IV IP and in the drinking water of experimental animals allowing for ready dosing. 9TB also has found use in studying hypoxia-regulated gene expression on cell survival using the tetracycline-inducible (tet-on) system where exposure to the inducing ligand doxycycline (dox) inhibited caspase-3 cleavage in mitochondria in Publications Powered by Bioz See more details on Bioz1) Zhu P. et al. “Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons†PLoS ONE. 2007; 2(6): e533.2) Halterman M.W. “An Improved Method for the Study of Apoptosis-Related Genes Using the Tet-On System†J. Biomol. Screen. 2011; 16(3): 332-3373) Jiang D. et al. "Airway Epithelial NF-kB Activation Promotes Mycoplasma pneumoniae Clearance in Mice" PLoS ONE. 2012; 7(12): e52969.4) Furutachi S. et al. "Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells" Nature Medicine 2015; doi:10.1038/nn.3989.5) Furlan G. et al. (2017). "Life-Long Neurogenic Activity of Individual Neural Stem Cells and Continuous Growth Establish an Outside-In Architecture in the Teleost Pallium." Current Biology 27(21): 3288-3301. DOI:10.1016/j.cub.2017.09.0526) Alonso S. B. et al. (2019). "An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants." The EMBO Journal 38: e98791. DOI:10.15252/embj.201798791
