- 中文名称
DBI, Human, BioAssay ELISA Kit (Acyl-CoA-binding Protein, ACBP, Diazepam-binding Inhibitor, Endozepine, EP)
- 英文名字
- DBI, Human, BioAssay ELISA Kit (Acyl-CoA-binding Protein, ACBP, Diazepam-binding Inhibitor, Endozepine, EP)
- 供应商
- USBiological
- 产品货号
- D3475-02Q
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Diazepam Binding Inhibitor/Acyl-coA binding protein (DBI, ACBP) is a highly conserved 10kD polypeptide which is expressed in various species range from yeast to mammals. As an inverse agonist for benzodiazepine receptors, DBI (ACBP) down-regulates inhibitory effects of GABA. It also has potential to induce anxiety. Found in central and peripheral tissues, DBI (ACBP) also participates in metabolism of steroids, which has been known to partially modify GABA receptor function in the CNS. In peripheral tissues, DBI(ACBP) plays regulatory roles in steroidogenesis. DBI (ACBP) levels have been reported to be decreased in the cerebrospinal fluid obtained from patients with Alzheimer disease.Intended Use:Diazepam Binding Inhibitor/ Acyl-coA binding protein (human DBI, ACBP) ELISA kit is to be used for the in vitro quantitative determination of human DBI(ACBP) in human serum, human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human DBI(ACBP). This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human DBI(ACBP) was calculated to be 25pg/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: mouse DBI (ACBP) and rat DBI(ACBP).Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human DBI (ACBP). Samples are pippetted into these wells. Nonbound DBI (ACBP) and other components of the sample should be removed by washing, then biotin- conjugated monoclonal antibody specific to DBI (ACBP) added. In order to quantitatively determine the amount of DBI (ACBP) present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured DBI (ACBP).Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP, (100x): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对DBI, Human, BioAssay ELISA Kit (Acyl-CoA-binding Protein, ACBP, Diazepam-binding Inhibitor, Endozepine, EP)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DBI, Human, BioAssay ELISA Kit (Acyl-CoA-binding Protein, ACBP, Diazepam-binding Inhibitor, Endozepine, EP)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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