Protein G is a protein that has the property of binging to immunoglobulins. It is a 65-kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
产品描述
Protein G purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein G purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. Protein G may offer improved purification compared to Protein A for certain immunoglobulin classes and for certain host organisms. The IgG is bound to an immobilized Protein G matrix, non-IgG impurities are washed away, lastly the IgG fraction is eluted and recovered. Rockland's Protein G purification process is ideal to recover a highly active IgG fraction from complex starting material.
产品形式
该PROTEIN G PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST19M的详细信息查看Rockland提供的产品说明书
保存建议
该PROTEIN G PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST19M的详细信息查看Rockland提供的产品说明书
