Osteoprotegerin (OPG) or osteoclast inhibitory factor (OCIF) is a glycoprotein of the tumor necrosis factor receptor superfamily encoded by the TNFRSF11B gene. OPG is synthesized as a monomer of 380 amino acids, assembled as a homodimer within the cell and then secreted mainly as a disulfide-linked homodimer into the extracellular compartment. OPG is produced by many different tissues and cell types including osteoblasts, breast tissue, vascular endothelial cells as well as B cells and dendritic cells in the immune system.
产品描述
Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum, plasma (EDTA, heparın, citrate)
Sample volume
20 µl / well
Assay time
4 h / 1 h / 30 min
Sensitivity
0.07 pmol/l (= 1.4 pg/ml)
Standard range
0 – 20 pmol/l (= 0 – 400 pg/ml)
Conversion factor
1 pg/ml = 0.05 pmol/l (MW: 19.9 kDa)
Specificity
Endogenous and recombinant human OPG.
Precision
In-between-run (n=12): ≤ 5 % CV
Within-run (n=5): ≤ 3 % CV
Cross-reactivity
The assay does not cross react with rat or mouse samples.
