- 中文名称
大内皮素Elisa试剂盒
- 英文名字
- Big Endothelin-1 ELISA Kit
- 供应商
- Biomedica Immunoassays
- 产品货号
- BI-20082H
- 产品报价
- ￥12x8tests
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- Big Endothelin-1 (BigET-1) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET-1), consisting of amino acids 1-21 (http://www.uniprot.org/uniprot/P05305). ET-1 is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly, it has a wide tissue distribution (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.511899). The cleavage of BigET-1 by the Endothelin-converting enzyme (ECE) converts BigET-1 to Endothelin (ET) and to a C‑terminal fragment.
The half-life of ET-1 (1-21) in plasma is less than one minute, which may preclude its use as a diagnostic marker, whereas clearance of the precursor peptide BigET-1 is much slower. As Big-Endothelin-1 is produced at amounts equimolar to ET-1 and has higher plasma stability, it is more suitable for diagnostics and monitoring of heart failure (Gergei et al., 2018). Hence, Big ET-1 has the same physiological role as ET-1 while being a more reliable marker.
- 产品描述
- Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum, EDTA plasma. citrate plasma, heparın plasma
Sample volume
50 µl / well
Assay time
4 h / 1 h / 30 min
Sensitivity
0.02 pmol/l (= 0.086 pg/ml)
Standard range
0 – 3 pmol/l (= 0 – 12.85 pg/ml)
Conversion factor
1 pmol/l = 4.283 pg/ml (MW: 4.283 kDa)
Precision
In-between-run (n=5): ≤ 5 % CV
Within-run (n=10): ≤ 4 % CV
Specificity
Endogenous and recombinant Big Endothelin-1 (Big ET-1 (1-38)).
Cross-reactivity
Human ET1/2/3 (1-21): <1%, human ET2 (1-37): <1%, human ET1/2 (1-38): <1%, human BigET1/2 (22-38) : <1%, human BigET2 (22-37) : <1%,
porcine BigET (1-39): 21%, rat BigET1 (1-39): 10%, Sarafotoxin: <1%
- 产品特点
- 保存建议
- +4°C
- 其他
- Biomedica Immunoassay(bmgrp)公司成立于1988年, 总部位于奥地利维也纳，主要致力于研究、开发和生产基于ELISA方法用于临床前研究相关标记物检测的工具。迄今为止Biomedica Immunoassay公司在心血管检测及骨代谢检测领域开发了一系列特色ELISA检测试剂盒，Biomedica Immunoassay的生产过程符合GMP/GLP指南，并符合ISO 9001:2015管理体系，有CE认证。Biomedica Immunoassay的产品根据FDA， EMEA和ICH指南进行验证，并具有验证报告。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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