NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor，IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS)，pro-inflammatory cytokines，and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
应用类型
WB, IHC
免疫原
A peptide corresponding to the area near the C-terminus of human transcription factor p65 (NFkB p65 subunit)
