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晚期氧化蛋白产物(AOPP)阳性标准品&试剂盒

发布者:艾美捷科技    发布时间:2021-06-15     
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晚期氧化蛋白产物.jpg


晚期氧化蛋白产物(AOPP,Advanced Oxidation Protein Products)是血浆蛋白被体内活性氧(ROS)攻击后形成的氧化修饰产物,是新近发现的一种具有促炎活性氧化应激指标。

 

1996年Witko-Sarsat等发现CRF病人血浆中AOPP水平明显增高,主要存在于白蛋白中,特征是酸性条件下在340nm有特异吸收峰。在体外用次氯酸(HOC1)与白蛋白作用可得到与CRF病人血浆中相似的AOPP。中性粒细胞活化时髓过氧化物酶(MPO)催化产生的HOC1可能修饰血浆蛋白而生成过量AOPP。AOPP在泌尿、呼吸、神经结缔组织、妇科和消化等全身多个系统相关疾病发生、发展中的关键作用均已被证实,其在众多疾病中的发病机制也逐渐明晰。

 

作为专业的生命科学医药原料供应商,Cell Biolabs中国区金牌代理,艾美捷科技为您推荐:晚期氧化蛋白产物(AOPP)阳性标准品&试剂盒

【1】标准品AOPP-HSA阳性标准品,AOPP-Human Serum Albumin
(晚期氧化蛋白产物-人血清白蛋白)
货号STA-319 (50ul, 7.5 mg/mL AOPP-HSA)
保存条件冰袋运输;收到货后,分装并保存在-20℃,以避免多次冷冻/解冻循环。
【2】试剂盒晚期氧化蛋白产物(AOPP)检测试剂盒
OxiSelect AOPP Assay Kit
货号STA-318 (200assays)
检测方法比色法
检测范围5 - 100 uM
说明书下载点击下载
实验原理未知的含AOPP样品或氯胺标准品首先与开始显色过程的测定反应引发剂混合。短暂孵育后,加入终止溶液,样品和标准品可以用标准比色板读取器读取。未知样品中的AOPP含量是通过与预定的氯胺标准曲线进行比较来确定的。
试剂盒组分#第一部分#
1. Chloramine Standard
2. 2Chloramine Reaction Initiator
3. Stop Solution
4. 10X Assay Diluent
#第二部分#
1. AOPP-HSA Positive Control
保存条件收到后,将AOPP-HSA阳性标准品分装并保存在-20℃,以避免多次冷冻/解冻循环。将所有其他试剂盒成分保存在4℃。

* 本产品仅适用于科研用途.

 

FAQ常见问题:

1.试剂盒适用于哪些类型的样品?

我们的晚期氧化蛋白产物(AOPP)检测试剂盒可用于检测高于检测限以上的任何样品类型,例如血清,血浆,细胞/组织裂解液(冻存样品:需在-80℃保存1年以内)。

 

2.应该如何准备裂解液样本?

我们建议在含有蛋白酶抑制剂的1XPBS中通过超声或匀浆裂解细胞或组织,以12000g离心10分钟,然后收集上清液作为裂解液。我们只推荐使用PBS制备裂解液,因为我们不知道裂解缓冲液是否会损坏AOPP。蛋白质浓度应通过蛋白质测定法确定,例如BCA或Bradford。蛋白质浓度将取决于样品,但建议在运行实验之前进行样品滴定以确定任何必要的稀释度,以便样品落在标准曲线内。

 

3.晚期氧化蛋白产物(AOPP)浓度如何展示结果?

样品 AOPP 的浓度以氯胺单位 (uM) 表示 (可参考下方发表文献)。

 

结果展示:

AOPP试剂盒标准曲线.jpg

AOPP-HSA阳性标准品.jpg

AOPP试剂盒标准曲线AOPP-HSA阳性标准品

 

发表文章:

  • Nukala, S.B. et al. (2021). Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension. Sci Rep. 11(1):5583. doi: 10.1038/s41598-021-85004-z (#STA-318).

  • AlMarabeh, S. et al. (2020). Chronic intermittent hypoxia impairs diuretic and natriuretic responses to volume expansion in rats with preserved low-pressure baroreflex control of the kidney. Am J Physiol Renal Physiol. doi: 10.1152/ajprenal.00377.2020 (#STA-318).

  • Xiao, L.L. et al. (2020). Using advanced oxidation protein products and ischaemia-modified albumin to monitor oxidative stress levels in patients with drug-induced liver injury. Sci Rep. 10(1):18128. doi: 10.1038/s41598-020-75141-2 (STA-318).

  • Bloomer, R.J. et al. (2020). Meta- bolic Health Outcomes Following Nine Months of Mild Caloric Restriction in Male Rats Adhering To a Western or Vegan Diet. J Altern Complement Integr Med. 6:097. doi: 10.24966/ACIM-7562/100097 (#STA-318).

  • Bigazzi, R. et al. (2019). Hypertension in High School Students: Genetic and Environmental Factors: The HYGEF Study. Hypertension. 75(1):71-78. doi: 10.1161/HYPERTENSIONAHA.119.13818 (#STA-318).

  • McAllister, M.J. et al. (2019). Acute coffee ingestion with and without medium chain triglycerides decreases blood oxidative stress markers and increases ketone levels. Can J Physiol Pharmacol. doi: 10.1139/cjpp-2019-0458 (#STA-318).

  • Ungurianu, A. et al. (2019). Preclinical and clinical results regarding the effects of a plant-based antidiabetic formulation versus well established antidiabetic molecules. Pharmacol Res. 150:104522. doi: 10.1016/j.phrs.2019.104522 (#STA-318).

  • Smith, C. et al. (2019). Chronic testosterone deprivation sensitizes the middle-aged rat brain to damaging effects of testosterone replacement. Neuroendocrinology. doi: 10.1159/000504445 (#STA-318).

  • Gryszczyńska, B. et al. (2019). Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment. BioMed Research International. 2019(7976043)1-9 pages. doi: 10.1155/2019/7976043 (#STA-318).

  • Morsy, M.D. et al. (2019). Protective effect of combined melatonin and α-tocopherol administration in spinal cord ischemia-reperfusion injury in rat. Int. J. Morphol. 37(2):428-437. doi: 10.4067/S0717-95022019000200428 (#STA-318).

  • Albatayneh, E.M. et al. (2019). Serum Oxidative-Antioxidative Status in Patients With Alkaptonuria. J Clin Med Res. 11(5):337-344. doi: 10.14740/jocmr3801 (#STA-318).

  • Shell, B. et al. (2019). Angiotensin Type 1a Receptors in the Median Preoptic Nucleus Support Intermittent Hypoxia-Induced Hypertension. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00393.2018 (#STA-318).

  • Bloomer, R. et al. (2018) Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women. Health. 10:960-970. doi: 10.4236/health.2018.107071 (#STA-318).

  • Sighinolfi, G. et al. (2018). AB0760 Advanced oxidation protein products in serum of patients with systemic sclerosis: a possible indicator of clinical evolution. Annals of the Rheumatic Diseases. 77:1516. doi: 10.1136/annrheumdis-2018-eular.6809 (#STA-318).

  • Wilson, E.N. et al. (2018). Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging. Physiol Behav. 189:64-73. doi: 10.1016/j.physbeh.2018.03.007 (#STA-318).

  • Gradinaru, D. et al. (2018). Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. doi: 10.1055/s-0043-123825 (#STA-318).

  • Hány?ová, S. et al. (2017). Elevated plasma levels of advanced oxidation protein products in Slovak multiple sclerosis patients: possible association with different disability states. Act Nerv Super Rediviva. 59(2): 45–50 (#STA-318).

  • Sun, S. et al. (2018). Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway. Redox Biol. 14:338-353. doi: 10.1016/j.redox.2017.09.011 (#STA-318).

  • Snyder, B. et al. (2017). Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol. Rep. doi: 10.14814/phy2.13258 (#STA-318).

  • Budzyń, M. et al. (2017). The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm. Hindawi BioMed Research International. doi: 10.1155/2017/2791082 (#STA-318).

  • Wan, X. et al. (2016). SIRT1-PGC1a-NFkB pathway of oxidative and inflammatory stress during Trypanosoma cruzi infection: benefits of SIRT1-targeted therapy in improving heart function in Chagas disease. PLoS Pathog. doi: 10.1371/journal.ppat.1005954 (#STA-318).

  • Gradinaru, D. et al. (2016). Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. doi:10.1007/s40520-016-0629-z (#STA-318).

  • Crone, L. B. et al. (2016). Impact of meal ingestion rate and caffeine coingestion on postprandial lipemia and oxidative stress following high-fat meal consumption. J Caffeine Res. doi:10.1089/jcr.2016.0004 (#STA-318).

  • Huemer, M. et al. (2016). Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency. J Inherit Metab Dis. doi:10.1007/s10545-016-9928-y (#STA-318).

  • Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582 (#STA-318).

  • ?ari?, B. et al. (2015). Oxidative stress impact on growth hormone secretion in the eye. Croat Med J. 56:326-333 (#STA-318).

  • Bloomer, R. J. et al. (2015). Comparison of a restricted and unrestricted vegan diet plan with a restricted omnivorous diet plan on health-specific measures. Healthcare.3:544-555 (#STA-318).

  • Jung, E. et al. (2015). Gemigliptin improves renal function and attenuates podocyte injury in mice with diabetic nephropathy. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.04.055 (#STA-318).

  • Thurmond, P. et al. (2015). Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia. Korean J Urol. 56:187-196 (#STA-318)..

  • Xie, Z. X. et al. (2014). Effect of GABA on oxidative stress in the skeletal muscles and plasma free amino acids in mice fed high-fat diet. J Anim Physiol Anim Nutr (Berl). doi: 10.1111/jpn.12254 (#STA-318).

 

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