- 中文名称
2-Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay Kit
- 英文名字
- 2-Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay Kit
- 供应商
- USBiological
- 产品货号
- T0700-89
- 产品报价
- ￥1/40Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- The TBARS assay was developed for the measurement of MDA production via lipid peroxidation and is a well-established and widely used method for assessment of oxidative damage. However, as typically performed, the traditional method for TBARS analysis is often criticized for lack of specificity and sensitivity. Indeed, currently-available TBARS assays require boiling the sample with strong acid which also generates interfering chromophores.This assay uses a room temperature TBARS method that eliminates the requirement for a high concentration of a strong acid and cuts reaction time in half. By running the assay at room temperature, low levels of interfering substances are generated. This results in more accurate, precise and reliable TBARS measurements.Advantages of this BioAssay Kit:Industry leading sensitivity (250nM) Enhanced MDA measurement with use of proprietary reagent formulation Reduced background signalShorter assay times; 15 minutes at RTUseful with any biological, nutraceutical or food sampleLess expensive than other methods now on the marketEliminates the requirement of boiling samplesCan use either cuvette or microplate formatsPrinciple of the Procedure:This assay is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25°C. One molecule of MDA reacts with 2 molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532nm, as shown in Figure 1.Kit Components:1. Indicator, 2-Thiobarbituric Acid, 2x1g2. Acid Reagent, 10% Acid Solution in Dimethylsulfoxide, 1x40ml3. MDA Standard, 10mM Malonaldehyde Tetrabutylammonium Salt, 1x100ul4. Microtiter plate, 2x96 wellsStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对2-Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay Kit 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到2-Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay Kit 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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