Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes， enzyme markers， biotin， colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% ， no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. （Free of Staphylococcus endotoxins and hemolysin）.
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大肠杆菌中重组表达
溶解建议
Sterile filtered colorless solution. The Protein-A protein solution contains 20mM Tris-HCl， pH-8 and 10% glycerol.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks.
Store， frozen at -20℃ for longer periods of time.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Avoid multiple freeze-thaw cycles.
