英文名字 ProteoSpin Inclusion Body Isolation Micro Kit
中文名称 内涵体提取试剂盒
供应商 Norgen Biotek
产品货号 NGB-10300
产品报价 ¥3762.91872/20preps
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产品新闻
背景资料 Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these sub-cellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time consuming and not cost effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.
产品描述 This kit provides everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.
产品特点 The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 礸 of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
保存建议 Cell Lysis Reagent和IB Solubilization Reagent储存在4°C。所有其他未开封的溶液应在室温下储存。一旦打开,溶液应储存在4°C,除了碱性和酸性结合缓冲液,应在室温下储存。具体保存情况收到产品后请按说明书存放。
产品说明书
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其他 Norgen Biotek目前的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。拥有超过二十个专利,作为一家成长型的公司,其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。此外,Norgen Biotek最近推出了100多款用于核酸诊断的试剂盒,包括从尿液、血浆和血清中诊断人类病原体的试剂盒,诊断食源性病原体的试剂盒和诊断植物病原体的试剂盒。这些试剂盒仅供研究使用,非常适合用于监测耐药病原体,流行病学研究,病原体现场监测和调查。Norgen Biotek还致力于为科研和临床试验提供高质量的分子诊断测试。

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