英文名字 Proteinase 3, Human, ELISA kit
中文名称 人 蛋白酶3 ELISA试剂盒
供应商 Hycult
产品货号 HK384-01
产品报价 10248/2 x 96 det
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产品新闻
背景资料 Proteinase 3 (PR3) is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. PR3 was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst.
产品描述 The Human Proteinase 3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human Proteinase 3. Biotinylated tracer antibody will bind to the captured Human Proteinase 3. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human Proteinase 3 standards (log). The Human Proteinase 3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
产品特点 Measurable concentration range of 0.63 to 40 ng/ml.
保存建议 Product should be stored at 4 ℃. Under recommended storage conditions, product is stable for one year.
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其他 荷兰Hycult Biotech的宗旨和策略是着重于天然免疫相关疾病的研究,为人类生活质量的提高谋福利.作为免疫学和细胞生物学领域的领导者,特别是天然免疫、炎症反应和细胞损伤等相关领域的领先者,Hycult Biotech公司不仅研发这些用于生物科学研究的产品,同时也致力于将这些科研产品转化为临床测试的研究.作为多家知名品牌的中国总代理以及中国区域代理,艾美捷与Hycult一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.

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