英文名字 CytoTell Green
中文名称 绿色活细胞荧光探针CytoTell Green
供应商 AAT Bioquest
产品货号 AAT-22253
产品报价 ¥1430/500Tests
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产品新闻
背景资料 Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations。 Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis。 However, there are a few severe problems associated with the use of CFSE for monitoring cell proliferation。 1)。 CFSE is highly toxic to cells。 CFSE indiscriminately reacts with all amino groups, thus changes many critical intracellular protein functions (such as cell membrane GPCRs); 2)。 CFSE has slow response and is inconvenient to use。 The CFSE fluorescence intensity of the 2nd generation cells is decreased more than 10 fold from the 1st generation。 You would have to wait for another generation to start the cell proliferation analysis。 3)。 Medium removal is required。 You would have to remove medium for cell analysis with a flow cytometer since CFSE reacts with medium components。 CytoTell Green is developed to eliminate these limitations。 It has distinct advantages over CFSE。 1)。 CytoTell Green has very minimal cytotoxicity since it does not react with proteins。 It is well retained in cells since the probe is designed to minimize the MDR interaction that generally pumps many cell stains out cells; 2)。 CytoTell Green exhibits much faster response and is more convenient to use than CFSE。 There is no fluorescence intensity gap between 1st and 2nd generation of cells。 As cells divide, CytoTell Green is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye; 3)。 There is no need to remove medium since CytoTell Green does not react with medium components; 4)。 CytoTell Green is more sensitive than CFSE。 Up to 9 generations may be visualized; 5)。 CytoTell Green is much more stable than CFSE。 CytoTell Green stock solution can be stored at room temperature for a few days。 CytoTell Green can also be used for long term tracking of labeled cells。 Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation。 Cells labeled with Cell CytoTell Green may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers。 CytoTell Green has a peak excitation of 520 nm and can be excited by the blue (488 nm) laser line, making it compatible with FITC filter set。
产品描述 绿色活细胞荧光探针CytoTell Green产品的光谱学相关参数为Ex(nm)=511/ Em(nm)525;其对应分子量大小为500。
产品形式 提供的绿色活细胞荧光探针CytoTell Green产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。
产品说明书
其他 美国AAT Bioquest Inc。(前身是ABD Bioquest,Inc。)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。作为AAT Bioquest Inc。的中国区域代理,艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品,包括显色,荧光和发光技术等全系列解决方案。

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