When proteins are conjugated to fluorescent organic dyes， fluorescence emission of the dye molecules is usually decreased， sometimes up to 5070%。 This quenching phenomenon has been acknowledged for decades， but as yet， there are no simple， practical methods to control the fluorescence of dyes conjugated to proteins， especially for dyes conjugated to immunoglobulins。 We offer this FluoroQuest fluorescence signal enhancing solution that might increase fluorescence up to 2。5-fold in cell imaging and flow analysis。 This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology， histochemistry， and cell biology。
