ProRed-derived protease substrates are colorless and non-fluorescent。 Cleavage of blocking protease-cleavable peptide residue by caspases generates the strongly red fluorescent ProRed that can be monitored fluorimetrically at ~620 nm with excitation of ~530 nm。 ProRed-derived caspase substrates are the most sensitive red indicators for the fluorimetric detection of various caspase activities。 This DEVD-ProRed substrate is specific for detecting caspases 3 and 7。
