EGF-D is most closely related to VEGF-C by virtue of the presence of N- and C-terminal extensions that are not found in other VEGF family members. In adult human tissues，VEGF-D mRNA is most abundant in heart，lung，skeletal muscle，colon，and small intestine. Analyses of VEGF-D receptor specificity revealed that VEGF-D is a ligand for both VEGF receptors （VEGFRs） VEGFR-2 （Flk1） and VEGFR-3 （Flt4） and can activate these receptors. However，VEGF-D does not bind to VEGFR-1. Expression of a truncated derivative of VEGF-D demonstrated that the receptor-binding capacities reside in the portion of the molecule that is most closely related in primary structure to other VEGF family members and that corresponds to the mature form of VEGF-C. In addition，VEGF-D is a mitogen for endothelial cells. The recombinant VEGF-D as the immunogen was expressed based on sequence from Genebank Accession# D89630，20-354 aa.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human VEGF-D （Cat.# 4643-10） can be used as a positive control. Other applications have not been determined.
免疫原
E. coli expressed human VEGF-D
来源宿主
兔
反应性
经检测验证，可与人种属来源的VEGF-D蛋白发生免疫反应.
保存建议
提供的VEGF-D抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该VEGF-D抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
