p53,a 53 kDa protein,binds to a DNA consensus sequence,the p53 response element,and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle,or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells,principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus,but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression,but may not always be necessary for apoptosis.
应用类型
Western blot analysis (0.5-4 ug/ml). It detects p53 in samples of human, mouse and rat origins. Other applications have not been determined.
免疫原
Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、狗、兔种属来源的p53蛋白发生免疫反应.
保存建议
提供的p53抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,请将该p53抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.