VEGF-B,a member of the VEGF family,is a potent growth and angiogenic cytokine. It promotes DNA synthesis in endothelial cells,regulates angiogenesis and vascular permeability,and inhibits apoptosis in certain smooth muscle cells and neurons. VEGF-B is expressed in all tissues except the liver. It forms cell surfaced-associated disulfide linked homodimers and can form heterodimers with VEGF-A. There are two known isoforms,formed by alternative splicing,which have been designated VEGF-B167 and VEGF-B186. Both forms have identical amino-terminal sequences encoding a “cysteine knot” like structural motif,but differ in their carboxyl-terminal domains. Both VEGF-B isoforms signal only through the VEGFR1 receptor. Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.
应用类型
Western Blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant human VEGF-B (Cat.# 4642-20) can be used as a positive control.
免疫原
E. coli-expressed recombinant human VEGF-B
来源宿主
兔
反应性
经检测验证,可与人种属来源的VEGF-B蛋白发生免疫反应
保存建议
提供的100 ug VEGF-B抗体产品为无色溶液其浓度为0.5 mg/ml溶解于包含有30%甘油, 0.5% BSA, 5 mM EDTA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该VEGF-B抗体产品置于-20 °C冷冻保存,建议分装使用以避免反复冻融.