Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis.
应用类型
Western blot analysis (0.5-4 ug/ml), Immunoprecipation (4-8 ug/ml), and Immunohistochemistry (10-20 ug/ml). Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ⍺ isoform of Bcl-2 on SDS-PAGE immunoblots.
免疫原
Synthetic peptide corresponding to the N-terminus of human Bcl-2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Bcl-2蛋白发生免疫反应.
保存建议
提供的Bcl-2抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bcl-2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.