Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-4 is also known as Ich-2,TX,and ICErel-II and can proteolytically processed by granzyme B. Over expression of caspase-4 induces apoptosis in Rat-1 and HeLa cells,which can be inhibited by CrmA and Bcl-2.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The affinity purified antibody detects the 43 kDa proform of caspase-4.
免疫原
Synthetic peptide surrounding amino acid 100 of human caspase-4
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Caspase-4蛋白发生免疫反应.
保存建议
提供的Caspase-4抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.