Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3,one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9.
免疫原
Synthetic peptide surrounding amino acid 290 of human caspase-9
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、仓鼠、牛、兔、猪种属来源的Caspase-9蛋白发生免疫反应.
保存建议
提供的Caspase-9抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-9抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.