Fas-associating death domain containing protein (FADD),also known as mediator of receptor induced toxicity (MORT1),is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas,an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA,a poxvirus protein that blocks both Fas- and TNF-induced cell death.
应用类型
Western blot analysis (1-4 ug/ml). The antibody detects 28 kDa protein, corresponding to the apparent molecular weight of FADD in SDS-PAGE However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.immunoblots from samples of human and mouse origins.
免疫原
Recombinant human FADD
来源宿主
小鼠
反应性
经检测验证,可与人、小鼠、狗种属来源的FADD蛋白 发生免疫反应.
保存建议
提供的FADD抗体 产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该FADD抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.