PARP,a 116 kDa nuclear poly (ADP-ribose) polymerase,is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215,which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
应用类型
Western blot analysis (1-4 ug/ml). The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP. However, the optimal concentrations should be determined individually.
免疫原
Synthetic peptide corresponding to caspase cleavage site in human PARP
来源宿主
兔
反应性
经检测验证,可与人种属来源的PARP蛋白发生免疫反应.
保存建议
提供的PARP抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞 的PBS缓冲液中.请将该PARP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.