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DNA甲基转移酶(DNMTs)活性/抑制分析试剂盒

发布者:艾美捷科技    发布时间:2018-05-25     
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DNA甲基转移酶(DNMTs)     

       DNA甲基化是哺乳动物基因组最常见的修饰方式,它是在DNA甲基转移酶(DNMTs)的催化下,以甲硫氨酸为供体,将甲基转移到胞嘧啶的第五个碳原子上。DNA甲基化是一个可逆的过程,在体内同时存在着主动或被动的去甲基化过程。     

 

       DNA甲基化主要发生在5'-CpG-3'的C上,在DNA甲基转移酶的催化下生成5-甲基胞嘧啶(5mC)。DNA甲基化的主要有三种酶:DNMT1,DNMT2,DNMT3a和DNMT3b以及他们的亚型。DNMT1是细胞中最重要的甲基转移酶,具有从头和维持甲基化的功能,DNMT1对胚胎的正常发育至关重要,并与基因组的不稳定性有关,DNMT1能与多种蛋白相互作用,参与基因的沉默,在许多肿瘤的发生和发展中都观察到DNMT1的过表达;DNMT2的生物功能到目前为止还不清楚;DNMT3a和DNMT3b是主要的从头甲基化的酶,许多原发肿瘤和转化细胞中都观察到了DNMT3B的过表达,DNMT3B在肿瘤发生的过程中对启动子的过甲基化有很重要的作用;DNMTs之间的作用也非常复杂。     

 

       近年来,DNA甲基化和人类疾病,尤其是和肿瘤之间关系引起研究人员的关注。许多研究发现,DNA甲基化模式的改变导致了肿瘤的发生。在很多肿瘤中都发现DNMTs的表达异常。这说明DNMTs在生物体内发挥着重要的作用。     艾美捷科技作为专业的生命科学领域解决方案供应商,为您推荐全球最畅销的DNA甲基转移酶(DNMTs)活性/抑制检测试剂盒:

  

 
产品名称及描述货号产品说明
DNMT Activity/Inhibition Assay Ultra Kit(比色法)P-3009详情
DNMT Activity/Inhibition Assay Ultra Kit(荧光法)P-3010详情

   

 

       DNA甲基转移酶活性/抑制试剂盒原理:  

       EpiQuik DNMT(DNA甲基转移酶)活性/抑制分析超级试剂盒是无放射性实验.通用的DNMT底物稳定的包被在孔板里,甲基转移酶把甲硫氨酸的甲基集团转移给胞嘧啶,甲基化DNA底物.甲基化的DNA能被抗-5-甲基化胞嘧啶的抗体所识别.比率或者大量甲基化的DNA与酶的活性成比例,然后类似Elisa的反应,比色法测定DNMT的活性,其吸收波长为450nm,DNMT的活性与光密度值成比例.  

   

       DNA甲基转移酶活性/抑制试剂盒试剂盒优势:  【全覆盖的适用种属】:哺乳动物(细胞,组织),植物,真菌,细菌等  

       1、快捷的实验操作,完成实验不超过3个小时45分钟;  

       2、安全创新性的比色实验不需要放射性物质,提取或者亲和层析;  

       3、超灵敏检测极限:核提取物低至0.5ug,纯化酶低至0.5ng,灵敏度是前任试剂盒的5倍;  

       4、最优化的抗体和增强液能高特异性的检测5-mc,与未甲基化的胞嘧啶没有交叉反应;  

       5、微孔板分析,既能适用手工操作,又能适用于高通量分析.

 

       实验流程与发表文章结果展示:

 

   DNA甲基转移酶(DNMTs)活性&抑制分析试剂盒实验结果   

 

  •        产品说明书:

 

       比色法:https://www.epigentek.com/docs/P-3009.pdf

       荧光法:https://www.epigentek.com/docs/P-3010.pdf     

 

       《已发表文章》

 

     

     

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