Nature同款,5羟甲基胞嘧啶(5hmC)羟化酶 TET活性分析

发布者:艾美捷科技发布时间:2021-04-01

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DNA胞嘧啶的表观遗传修饰通过调节基因转录在发育中起关键作用。目前研究最多的胞嘧啶修饰是5-甲基胞嘧啶(5mC),它与基因表达的抑制有关。在脱甲基化过程中,5mC氧化为连续的中间状态,包括5-羟甲基胞嘧啶(5hmC),5-甲酰基胞嘧啶(5fC)和5-羧基胞嘧啶(5caC)。在过去的十年中,研究表明,氧化形式的5mC,尤其是5hmC,除了在活性脱甲基过程中充当“中间体”外,还可能具有多种独立的功能。很多研究表明,5hmC 表达水 平与基因表达调控、多能干细胞分化、神经元发育、以及肿瘤发生都密切相关。


5hmC 表达水平


而参与其中甲基化循环的一个重要酶类,就是TET家族蛋白!人TET蛋白家族包含TET1、TET2和TET3这3个成员,所有TET蛋白都包含一个保守的双链β-螺旋结构域、一个富含半胱氨酸区和C端的核心催化结构域。TET1主要在胚胎干细胞中表达,TET2主要在造血系统中表达,TET3则在 小脑、皮质和海马体等神经系统中广泛表达。结果表明,基因组5hmC 水平与 TET 羟化酶活性相关。


那么如何检测5hmc羟化酶TET的活性呢?


作为专业的生命科学解决方案供应商,艾美捷为您推荐Nature同款方案:


产品名称5mC-Hydroxylase TET Activity/Inhibition Assay Kit
产品货号P-3086,比色法
P-9087,荧光法
产品原理在本实验中,甲基化底物被稳定地包埋在微孔板上。活性 TETs (客户提供)与底物结合,并将甲基化底物转化为羟甲基化产物。Tet- 转化的羟甲基化产物可以被特异性抗体识别。羟甲基化产物的比例或数量与酶的活性成正比,然后可以通过读取波长为450nm的吸光度或者荧光强度来对比分析。TET 酶的活性与测得的光强度成正比。
适用样本TET酶的细胞核提取物;纯化的TET酶(TETs, 1-3)
性能展示

5mC-Hydroxylase TET Activity/Inhibition Assay Kit性能展示

说明书下载点击下载


Nature文章结果:


DOI https://doi.org/10.1038/s41590-020-0764-8


1.TET酶活性分析使用P-3086; c. 使用艾美捷推荐的P-1032检测整体5hmc水平


更多客户的选择:


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